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whole human genome 4x44k v2 oligonucleotide microarray  (Agilent technologies)


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    Agilent technologies whole human genome 4x44k v2 oligonucleotide microarray
    Whole Human Genome 4x44k V2 Oligonucleotide Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/whole+human+genome+oligonucleotide+microarray/us12012616-494-8-11
    Average 90 stars, based on 1 article reviews
    whole human genome 4x44k v2 oligonucleotide microarray - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Highly Enhanced Expression of CD70 on Human T-Lymphotropic Virus Type 1-Carrying T-Cell Lines and Adult T-Cell Leukemia Cells
    Article Snippet: .. The gene expression in the HTLV-1-carrying T-cell lines S1T, MT-2, and M8166 was examined and compared with the expression in the HTLV-1-negative T-cell line MOLT-4 by Agilent whole human genome oligonucleotide microarray (4 44K slide format). ..

    Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer
    Article Snippet: .. Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software. ..

    Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer
    Article Snippet: .. Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software. ..

    Expressing:

    Article Title: Highly Enhanced Expression of CD70 on Human T-Lymphotropic Virus Type 1-Carrying T-Cell Lines and Adult T-Cell Leukemia Cells
    Article Snippet: .. The gene expression in the HTLV-1-carrying T-cell lines S1T, MT-2, and M8166 was examined and compared with the expression in the HTLV-1-negative T-cell line MOLT-4 by Agilent whole human genome oligonucleotide microarray (4 44K slide format). ..

    Microarray:

    Article Title: Highly Enhanced Expression of CD70 on Human T-Lymphotropic Virus Type 1-Carrying T-Cell Lines and Adult T-Cell Leukemia Cells
    Article Snippet: .. The gene expression in the HTLV-1-carrying T-cell lines S1T, MT-2, and M8166 was examined and compared with the expression in the HTLV-1-negative T-cell line MOLT-4 by Agilent whole human genome oligonucleotide microarray (4 44K slide format). ..

    Article Title: A health disparities study of MicroRNA-146a expression in prostate cancer samples derived from African American and European American patients
    Article Snippet: The RNA quantity was analyzed using the Nano Drop ND1000 (SOP N° TAL009) and RNA quality checked using a Bio-analyzer 2,100 (Agilent Technologies, CA, USA). .. Sample amplification was performed with 200 ng of total RNA using Agilent Technologies Quick Amp Labeling Kit One Color to generate complementary RNA (cDNA) for oligo microarrays. cDNA microarray analysis was processed using a Whole Human Genome Oligonucleotide Microarray (G4112A, 41,000 genes; Agilent Technologies, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer
    Article Snippet: .. Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software. ..

    Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer
    Article Snippet: .. Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software. ..

    Article Title: Gene expression profiling of human neural progenitor cells following the serum-induced astrocyte differentiation.
    Article Snippet: Neural stem cells (NSC) with self-renewal and multipotent properties could provide an ideal cell source for transplantation to treat spinal cord injury, stroke, and neurodegenerative diseases.. However, the majority of transplanted NSC and neural progenitor cells (NPC) differentiate into astrocytes in vivo under pathological environments in the central nervous system, which potentially cause reactive gliosis.. Because the serum is a potent inducer of astrocyte differentiation of rodent NPC in culture, we studied the effect of the serum on gene expression profile of cultured human NPC to identify the gene signature of astrocyte differentiation of human NPC.

    Article Title: Gene Expression Profiling of Embryonic Human Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra
    Article Snippet: Total RNA derived from adult human substantia nigra (n = 5) was purchased from Clontech (Palo Alto, CA, http://www.clontech.com ). .. Sample amplification was performed with 200 ng of total RNA using Agilent's Quick Amp Labeling Kit OnoColor to generate complementary RNA (cRNA) for oligo microarrays. cRNA was processed for microarray analysis on a Whole Human Genome Oligonucleotide Microarray (G4112A, 41,000 genes; Agilent Technologies, Santa Clara, CA, USA, and Illumina, USA), according to the manufacturer's instructions. .. The arrays were hybridized at 60°C for 17 h with the Tecan HS Pro hybridization station in the hybridization buffer containing fluorescence-labeled cRNA.

    Article Title: Human astrocytes express 14-3-3 sigma in response to oxidative and DNA-damaging stresses.
    Article Snippet: The 14-3-3 protein family consists of seven isoforms, most of which are expressed abundantly in neurons and glial cells, although the s isoform, a p53 target gene originally identified as an epithelium-specific marker, has not been identified in the human central nervous system.. Here, we show that human astrocytes in culture expressed 14-3-3s under stress conditions.. By Western blot, the expression of 14-3-3s, p53 and p21 was coordinately upregulated in astrocytes following exposure to hydrogen peroxide, 4-hydroxy-2-nonenal (4-HNE) or etoposide, a topoisomerase II inhibitor.

    Amplification:

    Article Title: A health disparities study of MicroRNA-146a expression in prostate cancer samples derived from African American and European American patients
    Article Snippet: The RNA quantity was analyzed using the Nano Drop ND1000 (SOP N° TAL009) and RNA quality checked using a Bio-analyzer 2,100 (Agilent Technologies, CA, USA). .. Sample amplification was performed with 200 ng of total RNA using Agilent Technologies Quick Amp Labeling Kit One Color to generate complementary RNA (cDNA) for oligo microarrays. cDNA microarray analysis was processed using a Whole Human Genome Oligonucleotide Microarray (G4112A, 41,000 genes; Agilent Technologies, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: Gene expression profiling of human neural progenitor cells following the serum-induced astrocyte differentiation.
    Article Snippet: Neural stem cells (NSC) with self-renewal and multipotent properties could provide an ideal cell source for transplantation to treat spinal cord injury, stroke, and neurodegenerative diseases.. However, the majority of transplanted NSC and neural progenitor cells (NPC) differentiate into astrocytes in vivo under pathological environments in the central nervous system, which potentially cause reactive gliosis.. Because the serum is a potent inducer of astrocyte differentiation of rodent NPC in culture, we studied the effect of the serum on gene expression profile of cultured human NPC to identify the gene signature of astrocyte differentiation of human NPC.

    Article Title: Gene Expression Profiling of Embryonic Human Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra
    Article Snippet: Total RNA derived from adult human substantia nigra (n = 5) was purchased from Clontech (Palo Alto, CA, http://www.clontech.com ). .. Sample amplification was performed with 200 ng of total RNA using Agilent's Quick Amp Labeling Kit OnoColor to generate complementary RNA (cRNA) for oligo microarrays. cRNA was processed for microarray analysis on a Whole Human Genome Oligonucleotide Microarray (G4112A, 41,000 genes; Agilent Technologies, Santa Clara, CA, USA, and Illumina, USA), according to the manufacturer's instructions. .. The arrays were hybridized at 60°C for 17 h with the Tecan HS Pro hybridization station in the hybridization buffer containing fluorescence-labeled cRNA.

    Article Title: Human astrocytes express 14-3-3 sigma in response to oxidative and DNA-damaging stresses.
    Article Snippet: The 14-3-3 protein family consists of seven isoforms, most of which are expressed abundantly in neurons and glial cells, although the s isoform, a p53 target gene originally identified as an epithelium-specific marker, has not been identified in the human central nervous system.. Here, we show that human astrocytes in culture expressed 14-3-3s under stress conditions.. By Western blot, the expression of 14-3-3s, p53 and p21 was coordinately upregulated in astrocytes following exposure to hydrogen peroxide, 4-hydroxy-2-nonenal (4-HNE) or etoposide, a topoisomerase II inhibitor.

    Labeling:

    Article Title: A health disparities study of MicroRNA-146a expression in prostate cancer samples derived from African American and European American patients
    Article Snippet: The RNA quantity was analyzed using the Nano Drop ND1000 (SOP N° TAL009) and RNA quality checked using a Bio-analyzer 2,100 (Agilent Technologies, CA, USA). .. Sample amplification was performed with 200 ng of total RNA using Agilent Technologies Quick Amp Labeling Kit One Color to generate complementary RNA (cDNA) for oligo microarrays. cDNA microarray analysis was processed using a Whole Human Genome Oligonucleotide Microarray (G4112A, 41,000 genes; Agilent Technologies, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: Gene expression profiling of human neural progenitor cells following the serum-induced astrocyte differentiation.
    Article Snippet: Neural stem cells (NSC) with self-renewal and multipotent properties could provide an ideal cell source for transplantation to treat spinal cord injury, stroke, and neurodegenerative diseases.. However, the majority of transplanted NSC and neural progenitor cells (NPC) differentiate into astrocytes in vivo under pathological environments in the central nervous system, which potentially cause reactive gliosis.. Because the serum is a potent inducer of astrocyte differentiation of rodent NPC in culture, we studied the effect of the serum on gene expression profile of cultured human NPC to identify the gene signature of astrocyte differentiation of human NPC.

    Article Title: Gene Expression Profiling of Embryonic Human Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra
    Article Snippet: Total RNA derived from adult human substantia nigra (n = 5) was purchased from Clontech (Palo Alto, CA, http://www.clontech.com ). .. Sample amplification was performed with 200 ng of total RNA using Agilent's Quick Amp Labeling Kit OnoColor to generate complementary RNA (cRNA) for oligo microarrays. cRNA was processed for microarray analysis on a Whole Human Genome Oligonucleotide Microarray (G4112A, 41,000 genes; Agilent Technologies, Santa Clara, CA, USA, and Illumina, USA), according to the manufacturer's instructions. .. The arrays were hybridized at 60°C for 17 h with the Tecan HS Pro hybridization station in the hybridization buffer containing fluorescence-labeled cRNA.

    Software:

    Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer
    Article Snippet: .. Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software. ..

    Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer
    Article Snippet: .. Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software. ..

    In Vitro:

    Article Title: Gene expression profiling of human neural progenitor cells following the serum-induced astrocyte differentiation.
    Article Snippet: Neural stem cells (NSC) with self-renewal and multipotent properties could provide an ideal cell source for transplantation to treat spinal cord injury, stroke, and neurodegenerative diseases.. However, the majority of transplanted NSC and neural progenitor cells (NPC) differentiate into astrocytes in vivo under pathological environments in the central nervous system, which potentially cause reactive gliosis.. Because the serum is a potent inducer of astrocyte differentiation of rodent NPC in culture, we studied the effect of the serum on gene expression profile of cultured human NPC to identify the gene signature of astrocyte differentiation of human NPC.

    Article Title: Human astrocytes express 14-3-3 sigma in response to oxidative and DNA-damaging stresses.
    Article Snippet: The 14-3-3 protein family consists of seven isoforms, most of which are expressed abundantly in neurons and glial cells, although the s isoform, a p53 target gene originally identified as an epithelium-specific marker, has not been identified in the human central nervous system.. Here, we show that human astrocytes in culture expressed 14-3-3s under stress conditions.. By Western blot, the expression of 14-3-3s, p53 and p21 was coordinately upregulated in astrocytes following exposure to hydrogen peroxide, 4-hydroxy-2-nonenal (4-HNE) or etoposide, a topoisomerase II inhibitor.

    Isolation:

    Article Title: Human astrocytes express 14-3-3 sigma in response to oxidative and DNA-damaging stresses.
    Article Snippet: The 14-3-3 protein family consists of seven isoforms, most of which are expressed abundantly in neurons and glial cells, although the s isoform, a p53 target gene originally identified as an epithelium-specific marker, has not been identified in the human central nervous system.. Here, we show that human astrocytes in culture expressed 14-3-3s under stress conditions.. By Western blot, the expression of 14-3-3s, p53 and p21 was coordinately upregulated in astrocytes following exposure to hydrogen peroxide, 4-hydroxy-2-nonenal (4-HNE) or etoposide, a topoisomerase II inhibitor.



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    qRT-PCR analysis of DEGs identified in the microarray. Notes: Comparison of DEGs between PCa xenografts from castrated mice and parental cell lines. ( A ) CWR22R vs CWR22. ( B ) VCaPR vs VCaP. Expression data are represented by a log ratio calculated by comparing ΔCq from the xenograft with ΔCq from the parent cells. ΔCq was calculated as the difference between Cq of the targeted genes and Cq of the endogenous control gene, β-actin. Using this method, 16/18 (89%) genes pulled from the microarray analysis were confirmed to be differentially expressed by qRT-PCR. Abbreviations: DEGs, differentially expressed genes; qRT-PCR, quantitative reverse transcription PCR; PCa, prostate cancer.

    Journal: Cancer Management and Research

    Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer

    doi: 10.2147/CMAR.S179467

    Figure Lengend Snippet: qRT-PCR analysis of DEGs identified in the microarray. Notes: Comparison of DEGs between PCa xenografts from castrated mice and parental cell lines. ( A ) CWR22R vs CWR22. ( B ) VCaPR vs VCaP. Expression data are represented by a log ratio calculated by comparing ΔCq from the xenograft with ΔCq from the parent cells. ΔCq was calculated as the difference between Cq of the targeted genes and Cq of the endogenous control gene, β-actin. Using this method, 16/18 (89%) genes pulled from the microarray analysis were confirmed to be differentially expressed by qRT-PCR. Abbreviations: DEGs, differentially expressed genes; qRT-PCR, quantitative reverse transcription PCR; PCa, prostate cancer.

    Article Snippet: Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software.

    Techniques: Quantitative RT-PCR, Microarray, Expressing